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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
28/09/2020 |
Actualizado : |
28/09/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
FERNÁNDEZ ,S.; FRAGA, M.; CASTELLS, M.; COLINA, R.; ZUNINO,P. |
Afiliación : |
MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ZUNINO. |
Título : |
Effect of the administration of Lactobacillus spp. strains on neonatal diarrhoea, immune parameters and pathogen abundance in pre-weaned calves. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Beneficial Microbes, 1 September 2020, Volume 11, Issue 5, Pages 477-488. Doi: https://doi.org/10.3920/BM2019.0167 |
DOI : |
10.3920/BM2019.0167 |
Idioma : |
Inglés |
Contenido : |
Abstract:
Neonatal calf diarrhoea is one of the challenges faced by intensive farming, and probiotics are considered a promising approach to improve calves' health. The objective of this study was to evaluate the effect of potential probiotic lactobacilli on new-born dairy calves' growth, diarrhoea incidence, faecal score, cytokine expression in blood cells, immunoglobulin A (IgA) levels in plasma and faeces, and pathogen abundance in faeces. Two in vivo assays were conducted at the same farm in two annual calving seasons. Treated calves received one daily dose of the selected lactobacilli (Lactobacillus reuteri TP1.3B or Lactobacillus johnsonii TP1.6) for 10 consecutive days. A faecal score was recorded daily, average daily gain (ADG) was calculated, and blood and faeces samples were collected. Pathogen abundance was analysed by absolute qPCR in faeces using primers directed at Salmonella enterica, rotavirus, coronavirus, Cryptosporidium parvum and three Escherichia coli virulence genes (eae, clpG and Stx1). The faecal score was positively affected by the administration of both lactobacilli strains, and diarrhoea incidence was significantly lower in treated calves. No differences were found regarding ADG, cytokine expression, IgA levels and pathogen abundance. Our findings showed that oral administration of these strains could improve gastrointestinal health, but results could vary depending on the calving season, which may be related to pathogen seasonality and other environmental effects. MenosAbstract:
Neonatal calf diarrhoea is one of the challenges faced by intensive farming, and probiotics are considered a promising approach to improve calves' health. The objective of this study was to evaluate the effect of potential probiotic lactobacilli on new-born dairy calves' growth, diarrhoea incidence, faecal score, cytokine expression in blood cells, immunoglobulin A (IgA) levels in plasma and faeces, and pathogen abundance in faeces. Two in vivo assays were conducted at the same farm in two annual calving seasons. Treated calves received one daily dose of the selected lactobacilli (Lactobacillus reuteri TP1.3B or Lactobacillus johnsonii TP1.6) for 10 consecutive days. A faecal score was recorded daily, average daily gain (ADG) was calculated, and blood and faeces samples were collected. Pathogen abundance was analysed by absolute qPCR in faeces using primers directed at Salmonella enterica, rotavirus, coronavirus, Cryptosporidium parvum and three Escherichia coli virulence genes (eae, clpG and Stx1). The faecal score was positively affected by the administration of both lactobacilli strains, and diarrhoea incidence was significantly lower in treated calves. No differences were found regarding ADG, cytokine expression, IgA levels and pathogen abundance. Our findings showed that oral administration of these strains could improve gastrointestinal health, but results could vary depending on the calving season, which may be related to pathogen seasonality and other envir... Presentar Todo |
Palabras claves : |
CALVES; DIARREA; DIARREA NEONATAL; DIARRHOEA; LACTOBACILLUS SPP; PLATAFORMA SALUD ANIMAL; PROBIÓTICOS; PROBIOTICS. |
Asunto categoría : |
E16 Enfermedades de los animales |
Marc : |
LEADER 02407naa a2200277 a 4500 001 1061350 005 2020-09-28 008 2020 bl uuuu u00u1 u #d 024 7 $a10.3920/BM2019.0167$2DOI 100 1 $aFERNÁNDEZ ,S. 245 $aEffect of the administration of Lactobacillus spp. strains on neonatal diarrhoea, immune parameters and pathogen abundance in pre-weaned calves.$h[electronic resource] 260 $c2020 520 $aAbstract: Neonatal calf diarrhoea is one of the challenges faced by intensive farming, and probiotics are considered a promising approach to improve calves' health. The objective of this study was to evaluate the effect of potential probiotic lactobacilli on new-born dairy calves' growth, diarrhoea incidence, faecal score, cytokine expression in blood cells, immunoglobulin A (IgA) levels in plasma and faeces, and pathogen abundance in faeces. Two in vivo assays were conducted at the same farm in two annual calving seasons. Treated calves received one daily dose of the selected lactobacilli (Lactobacillus reuteri TP1.3B or Lactobacillus johnsonii TP1.6) for 10 consecutive days. A faecal score was recorded daily, average daily gain (ADG) was calculated, and blood and faeces samples were collected. Pathogen abundance was analysed by absolute qPCR in faeces using primers directed at Salmonella enterica, rotavirus, coronavirus, Cryptosporidium parvum and three Escherichia coli virulence genes (eae, clpG and Stx1). The faecal score was positively affected by the administration of both lactobacilli strains, and diarrhoea incidence was significantly lower in treated calves. No differences were found regarding ADG, cytokine expression, IgA levels and pathogen abundance. Our findings showed that oral administration of these strains could improve gastrointestinal health, but results could vary depending on the calving season, which may be related to pathogen seasonality and other environmental effects. 653 $aCALVES 653 $aDIARREA 653 $aDIARREA NEONATAL 653 $aDIARRHOEA 653 $aLACTOBACILLUS SPP 653 $aPLATAFORMA SALUD ANIMAL 653 $aPROBIÓTICOS 653 $aPROBIOTICS 700 1 $aFRAGA, M. 700 1 $aCASTELLS, M. 700 1 $aCOLINA, R. 700 1 $aZUNINO,P. 773 $tBeneficial Microbes, 1 September 2020, Volume 11, Issue 5, Pages 477-488. Doi: https://doi.org/10.3920/BM2019.0167
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INIA La Estanzuela (LE) |
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| Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
15/01/2020 |
Actualizado : |
15/01/2020 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
CASTELLS, M.; GIANNITTI, F.; CAFFARENA, D.; CASAUX, M.L.; SCHILD, C.; CASTELLS, D.; RIET-CORREA, F.; VICTORIA, M.; PAREÑO, V.; COLINA, R. |
Afiliación : |
MATÍAS CASTELLS BAUER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Laboratorio de Virología Molecular CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República Salto Uruguay.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DANIEL CASTELLS, Centro de Investigación y Experimentación Dr. Alejandro Gallinal, Secretariado Uruguayo de la Lana, Ruta 7 km 140, Cerro Colorado, Florida, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MATÍAS VISTORIA, Laboratorio de Virología Molecular CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República Salto Uruguay; VIVIANA PAREÑO, Sección de Virus Gastroentéricos, Instituto de Virología, CICV y A, INTA Castelar Buenos Aires Argentina.; RODNEY COLINA, Laboratorio de Virología Molecular, CENUR Litoral Norte, Centro Universitario de Salto, Universidad de la República, Rivera 1350, Salto, Uruguay. |
Título : |
Bovine coronavirus in Uruguay: genetic diversity, risk factors and transboundary introductions from neighboring countries. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
Archives of Virology,2019 Nov, Vol. 164 (11), p. 2715-2724. DOI: https://10.1007/s00705-019-04384-w |
ISSN : |
1432-8798 (online) |
DOI : |
10.1007/s00705-019-04384-w |
Idioma : |
Inglés |
Notas : |
Article history:Received 26 June 2019/Accepted 30 July 2019/Published 27 August 2019. |
Contenido : |
Abstract: Bovine coronavirus (BCoV) is a recognized cause of severe neonatal calf diarrhea, with a negative impact on animal welfare, leading to economic losses to the livestock industry. Cattle production is one of the most important economic sectors in Uruguay. The aim of this study was to determine the frequency of BCoV infections and their genetic diversity in Uruguayan calves and to describe the evolutionary history of the virus in South America. The overall detection rate of BCoV in Uruguay was 7.8% (64/824): 7.7% (60/782) in dairy cattle and 9.5% (4/42) in beef cattle. The detection rate of BCoV in samples from deceased and live calves was 10.0% (6/60) and 7.6% (58/763), respectively. Interestingly, there was a lower frequency of BCoV detection in calves born to vaccinated dams (3.3%, 8/240) than in calves born to unvaccinated dams (12.2%, 32/263) (OR: 4.02, 95%CI: 1.81-8.90; p = 0.00026). The frequency of BCoV detection was higher in colder months (11.8%, 44/373) than in warmer months (1.5%, 3/206) (OR: 9.05, 95%CI: 2.77-29.53, p = 0.000013). Uruguayan strains grouped together in two different lineages: one with Argentinean strains and the other with Brazilian strains. Both BCoV lineages were estimated to have entered Uruguay in 2013: one of them from Brazil (95%HPD interval: 2011-2014) and the other from Argentina (95%HPD interval: 2010-2014). The lineages differed by four amino acid changes, and both were divergent from the Mebus reference strain. Surveillance should be maintained to detect possible emerging strains that can clearly diverge at the antigenic level from vaccine strains. MenosAbstract: Bovine coronavirus (BCoV) is a recognized cause of severe neonatal calf diarrhea, with a negative impact on animal welfare, leading to economic losses to the livestock industry. Cattle production is one of the most important economic sectors in Uruguay. The aim of this study was to determine the frequency of BCoV infections and their genetic diversity in Uruguayan calves and to describe the evolutionary history of the virus in South America. The overall detection rate of BCoV in Uruguay was 7.8% (64/824): 7.7% (60/782) in dairy cattle and 9.5% (4/42) in beef cattle. The detection rate of BCoV in samples from deceased and live calves was 10.0% (6/60) and 7.6% (58/763), respectively. Interestingly, there was a lower frequency of BCoV detection in calves born to vaccinated dams (3.3%, 8/240) than in calves born to unvaccinated dams (12.2%, 32/263) (OR: 4.02, 95%CI: 1.81-8.90; p = 0.00026). The frequency of BCoV detection was higher in colder months (11.8%, 44/373) than in warmer months (1.5%, 3/206) (OR: 9.05, 95%CI: 2.77-29.53, p = 0.000013). Uruguayan strains grouped together in two different lineages: one with Argentinean strains and the other with Brazilian strains. Both BCoV lineages were estimated to have entered Uruguay in 2013: one of them from Brazil (95%HPD interval: 2011-2014) and the other from Argentina (95%HPD interval: 2010-2014). The lineages differed by four amino acid changes, and both were divergent from the Mebus reference strain. Surveillance shou... Presentar Todo |
Palabras claves : |
CATTLE DISEASES; CORONAVIRUS; PLATAFORMA DE SALUD ANIMAL. |
Thesagro : |
EPIDEMIOLOGIA. |
Asunto categoría : |
E16 Enfermedades de los animales |
Marc : |
LEADER 02667naa a2200313 a 4500 001 1060611 005 2020-01-15 008 2019 bl uuuu u00u1 u #d 022 $a1432-8798 (online) 024 7 $a10.1007/s00705-019-04384-w$2DOI 100 1 $aCASTELLS, M. 245 $aBovine coronavirus in Uruguay$bgenetic diversity, risk factors and transboundary introductions from neighboring countries.$h[electronic resource] 260 $c2019 500 $aArticle history:Received 26 June 2019/Accepted 30 July 2019/Published 27 August 2019. 520 $aAbstract: Bovine coronavirus (BCoV) is a recognized cause of severe neonatal calf diarrhea, with a negative impact on animal welfare, leading to economic losses to the livestock industry. Cattle production is one of the most important economic sectors in Uruguay. The aim of this study was to determine the frequency of BCoV infections and their genetic diversity in Uruguayan calves and to describe the evolutionary history of the virus in South America. The overall detection rate of BCoV in Uruguay was 7.8% (64/824): 7.7% (60/782) in dairy cattle and 9.5% (4/42) in beef cattle. The detection rate of BCoV in samples from deceased and live calves was 10.0% (6/60) and 7.6% (58/763), respectively. Interestingly, there was a lower frequency of BCoV detection in calves born to vaccinated dams (3.3%, 8/240) than in calves born to unvaccinated dams (12.2%, 32/263) (OR: 4.02, 95%CI: 1.81-8.90; p = 0.00026). The frequency of BCoV detection was higher in colder months (11.8%, 44/373) than in warmer months (1.5%, 3/206) (OR: 9.05, 95%CI: 2.77-29.53, p = 0.000013). Uruguayan strains grouped together in two different lineages: one with Argentinean strains and the other with Brazilian strains. Both BCoV lineages were estimated to have entered Uruguay in 2013: one of them from Brazil (95%HPD interval: 2011-2014) and the other from Argentina (95%HPD interval: 2010-2014). The lineages differed by four amino acid changes, and both were divergent from the Mebus reference strain. Surveillance should be maintained to detect possible emerging strains that can clearly diverge at the antigenic level from vaccine strains. 650 $aEPIDEMIOLOGIA 653 $aCATTLE DISEASES 653 $aCORONAVIRUS 653 $aPLATAFORMA DE SALUD ANIMAL 700 1 $aGIANNITTI, F. 700 1 $aCAFFARENA, D. 700 1 $aCASAUX, M.L. 700 1 $aSCHILD, C. 700 1 $aCASTELLS, D. 700 1 $aRIET-CORREA, F. 700 1 $aVICTORIA, M. 700 1 $aPAREÑO, V. 700 1 $aCOLINA, R. 773 $tArchives of Virology,2019 Nov, Vol. 164 (11), p. 2715-2724. DOI: https://10.1007/s00705-019-04384-w
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